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By targeting hypoxia of TME immunosuppression, MnO 2 NPs can exert their peroxide-like activity to react with tumor endogenous H 2 O 2 , which could generate in situ O 2 to relieve TME hypoxia to remit the immune suppression in solid tumors (33, 81)

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For analysis performed with the CytExpert, compensation matrices and gating settings were set based on signals in all measured channels, and the same gates and matrices were used for all samples

However, cell-free DNA (cfDNA) analysis, which overcomes these limitations, can provide direct evidence of residual disease and can be used as a minimally invasive detection method for patients with CRC