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The intervention led to promoter hypermethylation of critical genes involved in Wnt signaling and inflammation, pathways fundamentally implicated in breast carcinogenesis

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On the other hand, some data indicate that a high ascorbic acid (1 mM) concentration may lead to a disturbed Keap1/Nrf2/ARE pathway activation [85]

Sensitive and accurate: linear detection range 0.4 - 100 M in 96-well plate Simple and convenient: mix the DTNB reagent with sample, remove protein precipitates for proteinaceous samples, add a second reagent and read the optical density Low interference: amino acids and common buffers do not interfere Direct assays: reduced glutathione in whole blood, plasma, serum, urine, tissue and cell extracts Drug discovery/pharmacology: effects of drugs on glutathione metabolism Note: The below protocol is a sample protocol