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One g of RNA was reverse transcribed into cDNA using the Transcriptor First Strand cDNA synthesis kit (Roche, Indianapolis, IN, USA) and subjected to quantitative PCR using the SYBR-Green Supermix (Roche, Indianapolis, IN, USA) with the ABI Stepone Plus real-time PCR detection system (Applied Biosystems, Foster City, CA, USA)

Lindner M, Gosewisch A, Eilles E, et al

The sensation of pain was similar between formulations containing phenol (3 mg/ml) and benzyl alcohol (39 mg/ml), whereas m -cresol (9 mg/ml) was associated with more painful injections than benzyl alcohol

These bacteria can either be live or inactive