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Free Radical Biol Med 37:287303 Ganesan K, Kumar KS, Rao PVS (2011) Comparative assessment of antioxidant activity in three edible species of green seaweed, Enteromorpha from Okha, Northwest coast of India

Then the COCs were washed three times in DPBS, twice in the maturation medium (the medium was TCM-199 supplemented with 10% FBS, both FBS and TCM-199 were purchased from Thermo Fisher Scientific), and cultured in an incubator with a CO 2 concentration of 5% at 38.5C for 27 h