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M., Limbutara, K., Knepper, M

Additionally, conditional lethal switches or auxotrophic markers can be introduced to design the engineered bacteria to survive only in the presence of specific synthetic nutrients [206]

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To determine the anti-proliferative effect of ACE, we conducted a colony formation assay and 5-ethynyl-20-deoxyuridine (EdU) assay and revealed that ACE significantly reduced colony formation efficiency and cell growth (Fig