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Supporting optimal glutathione levels may help maintain efficient liver function

Compared with diabetic rats, the left ventricular hemodynamic parameters and myocardial ultrastructure changes (myocardial hypertrophy and fibrosis) are obviously normalized in diabetic animals treated with NaHS, which may be associated with upregulation of cardiac myosin light chain kinase (MLCK) and downregulation of serum creatine kinase MB isozyme (CK-MB) (Yang et al., 2016a)

2024;143(Pt 2):113470

After incubation for 30 min, 50 l of the mixture were transferred to the wells of a black F16 Fluoronunc module (ThermoFisher Scientific, Nunc) containing 50 l of 10 mM fluorogenic thrombin substrate (Boc-Asp(OBzl)-Pro-Arg-AMC) and thrombin catalyzed substrate hydrolysis was monitored at ex of 360 nm and em of 460 nm using a Synergy 2 microplate reader (BioTek, Bad Friedrichshall, Germany)