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To elucidate the lack of a statistically significant increase in brain tissue BDNF levels in the REM sleep deprivation group compared to the control sleep deprivation group and control group, future studies could employ a multiplatform sleep deprivation model, incorporating various cage configurations and disk sizes

Three recombinant strains harbouring two copies of gshF gene relative to that of W303-1b/F (Additional file 1: Table S8) were obtained and compared for their GSH production with W303-1b/F