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The oxidation of NADPH to NADP+ is accompanied by a decrease in absorbance at 340 nm

10.1097/01.smj.0000202698.25909.97 45

Plant Physiol Biochem 57:1522 Molla MR, Ali MR, Hasanuzzaman M, Al-Mamun MH, Ahmed A, Nazim-Ud-Dowla MAN, Rohman MM (2014) Exogenous proline and betaine-induced upregulation of glutathione transferase and glyoxalase I in lentil ( Lens culinaris ) under drought stress

The review found that oral and topical formulations demonstrate significant but variable melanin reduction with minimal side effects, while intravenous glutathione carries serious safety concerns including anaphylaxis and hepatotoxicity