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Step 3: An acid/base reaction

Before initiate the experimental sets, the antioxidant activity of Ab aqueous extract was confirmed by the in vitro assay for total antioxidant activity (TAC) (data not shown)

A prospective observational study found that SCFA producing bacteria and fecal SCFA levels in AIS patients were significantly reduced, suggesting that SCFA is a marker of the severity and prognosis of AIS, and may also be a potential therapeutic target (132)

Tissue homogenization: Frozen tissue of brain was homogenized in 9 volumes of ice-cold tissue lysis buffer containing 150 mM sodium chloride, 1.0% NP-40, 50 mM Tris, pH 8.0 and 1 mM PMSF (phenylmethane sulfonylfluoride) with protease inhibitors (Sigma, Singapore)