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For instance, in a study where a chimeric AARS mutant was developed by combining the tRNA binding domain of PylRS with the catalytic domain of Ec HisRS, researchers utilized a chimeric tRNA based on tRNA Pyl (Figure 9) This chimeric tRNA, featuring only a partial replacement of the acceptor stem with Ec tRNA His , facilitated recognition and UAA charging by the chimeric AARS

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