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Namely, its reduced and active form (GSH), significantly increased over time (Fig 7B

The method of any of claims 4 to 7, wherein a nascent bifunctional complex is dispensed in separate reaction compartments and subsequently exposed to one or more different chemical entities in each of a plurality of the reaction compartments, wherein the different chemical entities react with at least another chemical entity or with a chemical reaction site of the nascent bifunctional complex, and wherein an oligonucleotide tag identifying the different chemical entity is added enzymatically or chemically, and/or wherein the polymerase mediated primer extension, when this step is included in the method, results in the formation of a diversification-sequence capable of diversifying otherwise in-distinguishable tag combinations, thereby identifying tag combination sequences arising from a single tag combination, and/or wherein at least about 50% of the molecules have less than 6 hydrogen bond acceptors and/or less than 6 hydrogen bond donors, and/or a LogP value of less than 6, and/or wherein the identifier oligonucleotide has a length of from 6 to about 300 nucleotides

thus, having tangible options in current practice may shift attitudes beyond what was previously theoretical

With the increase of GR amount, the rate of the initial cleavage reaction is accelerated and more GSH is yielded as revealed by the higher fluorescent intensity after the completion of the enzymatic reaction (Figures S9S12)