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In vitro ALI culture (DAPT stimulation) Tracheal cells (P1) were expanded a second time on rattail collagen-coated dishes in PneumaCult Expansion Plus Medium (PEP)/RI and harvested by trypsinization with FBS neutralization

Turning to the highest shares, among men aged 3554 years in Bulgaria and aged 4554 years in Latvia, more than 45 % were daily smokers

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