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Assessment of the Change in the Brain and Veins Volume Proportional to the Change in the Brain and Veins Surface Area As described previously [17,18,19,20,21,22,23], the presentation of the brain (in vivo and ex vivo) and peripheral veins (superior mesenteric vein, inferior vena cava and azygos vein) was recorded in deeply anaesthetized rats that had undergone laparotomy or complete calvariectomy using a camera attached to a VMS-004 Discovery Deluxe USB microscope (Veho, Dayton, OH, USA)

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At normal intracellular concentrations, ROS acts as a redox signaling messenger, required for a number of pivotal cellular functions including cell differentiation, proliferation, growth, and apoptosis [3]

Lysate was diluted in 1 mL chloroform:methanol (2:1 v/v) and vortexed for 5 min at max speed