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Firstly, activated mTOR reduces the antioxidant activity of mesangial cells in the glomerulus, promoting the production of ROS

Phosphorylation assay was performed in the kinase buffer (0.6% (w/vol) CHAPS, 150 mM NaCl, 25 mM Hepes pH 7.5, 10 mM MgCl 2 and 1 mM MnCl 2 supplemented with 0.25 mM ATP) and incubated at 25 C for 5 min

Additional information Peer review information Nature Reviews Cancer thanks S.A

Pulmonary edema may result from catecholamine-induced myocarditis, myocardial ischemia due to coronary vasoconstriction, and direct cardiotoxin effects on the myocardium (103106)