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doi: 10.1007/s11240-017-1320-6 28 EulgemT.SomssichI

Similarly, Klotho was related to IGF1 signalling inhibition, thus to neuroprotection in Alzheimers disease mouse model 36

Protein expression induction, using 1 mM IPTG (Sigma), was done in BL21(DE3) cells starting with an optical density (OD) 600 of 0.6 in standard lysogeny broth (LB) medium